Review



cellcyte studio software  (Cytena Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Cytena Inc cellcyte studio software
    <t>Cellcyte</t> X live imaging of IM3 immortalized vascular smooth muscles over 7 days in (A) DMEM, 10% FBS, (B) calcification medium CM (DMEM, 10% FBS, adjusted to 4.3 mM Ca and 3 mM Pi), and (C) endothelial calcification medium ECM-2 (endothelial base medium EBM-2, 10% FBS, adjusted to 4.3 mM total Ca and 3.0 mM total Pi). Movies are time-lapse with frames acquired every three hours. C.Live Tox green, a nuclear stain demarcating dead cells, is visible in green. (D) Quantification of threshold object count in the green channel, indicating the number of dead cells per field of view. Each data series consists of four analyzed fields of view of the same condition. Each measurement was performed in quadruplicate (n = 4). Error bars depict standard deviation.
    Cellcyte Studio Software, supplied by Cytena Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellcyte studio software/product/Cytena Inc
    Average 86 stars, based on 1 article reviews
    cellcyte studio software - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "Cell-based calcification assays – magnesium overrules calcium phosphate saturation in overall outcome"

    Article Title: Cell-based calcification assays – magnesium overrules calcium phosphate saturation in overall outcome

    Journal: bioRxiv

    doi: 10.64898/2025.12.23.696208

    Cellcyte X live imaging of IM3 immortalized vascular smooth muscles over 7 days in (A) DMEM, 10% FBS, (B) calcification medium CM (DMEM, 10% FBS, adjusted to 4.3 mM Ca and 3 mM Pi), and (C) endothelial calcification medium ECM-2 (endothelial base medium EBM-2, 10% FBS, adjusted to 4.3 mM total Ca and 3.0 mM total Pi). Movies are time-lapse with frames acquired every three hours. C.Live Tox green, a nuclear stain demarcating dead cells, is visible in green. (D) Quantification of threshold object count in the green channel, indicating the number of dead cells per field of view. Each data series consists of four analyzed fields of view of the same condition. Each measurement was performed in quadruplicate (n = 4). Error bars depict standard deviation.
    Figure Legend Snippet: Cellcyte X live imaging of IM3 immortalized vascular smooth muscles over 7 days in (A) DMEM, 10% FBS, (B) calcification medium CM (DMEM, 10% FBS, adjusted to 4.3 mM Ca and 3 mM Pi), and (C) endothelial calcification medium ECM-2 (endothelial base medium EBM-2, 10% FBS, adjusted to 4.3 mM total Ca and 3.0 mM total Pi). Movies are time-lapse with frames acquired every three hours. C.Live Tox green, a nuclear stain demarcating dead cells, is visible in green. (D) Quantification of threshold object count in the green channel, indicating the number of dead cells per field of view. Each data series consists of four analyzed fields of view of the same condition. Each measurement was performed in quadruplicate (n = 4). Error bars depict standard deviation.

    Techniques Used: Imaging, Muscles, Staining, Standard Deviation

    Cellcyte-X live imaging of IM3 immortalized vascular smooth muscle cells over 7 days in DMEM, 10% FBS, with 10 mM added Ca and Pi and (A) 1.25 mM, (B) 2.5 mM, (C) 5 mM, and 10 mM added Mg. Fetuin-A-mRuby staining of calcification is shown in red. Movement of living cells can be seen in , but not in C. The morphology of the calcification can be seen to change with increasing Mg. Lower concentrations of Mg exhibited more rapid onset of calcification along cell boundaries, while higher concentrations exhibited delayed onset followed by sudden and sheet-like calcification across the cell layer. At the highest concentration, 10 mM added Mg, no calcification was observed.
    Figure Legend Snippet: Cellcyte-X live imaging of IM3 immortalized vascular smooth muscle cells over 7 days in DMEM, 10% FBS, with 10 mM added Ca and Pi and (A) 1.25 mM, (B) 2.5 mM, (C) 5 mM, and 10 mM added Mg. Fetuin-A-mRuby staining of calcification is shown in red. Movement of living cells can be seen in , but not in C. The morphology of the calcification can be seen to change with increasing Mg. Lower concentrations of Mg exhibited more rapid onset of calcification along cell boundaries, while higher concentrations exhibited delayed onset followed by sudden and sheet-like calcification across the cell layer. At the highest concentration, 10 mM added Mg, no calcification was observed.

    Techniques Used: Imaging, Staining, Concentration Assay

    Cellcyte X live fluorescence quantification of IM3 immortalized vascular smooth muscle cells over 7 days using fluorescence-labelled fetuin-A-mRuby3. Cells were cultured in DMEM, 10% FBS, with 10 mM additional Ca and either 10 mM (A-B) or 5 mM additional Pi (C-D) with additional Mg as listed in the figure legend. (A, C): Complete time course of live imaging data. (B, D): First 72 hours of time course, zoomed for readability. Each data series consists of fluorescence quantification of four fields of view per condition with error bars shown (n = 4). Fluorescence intensity of fetuin-A-mRuby3 indicates degree of calcification, with background intensity measured in DMEM. Error bars depict standard deviation with very small errors not visible.
    Figure Legend Snippet: Cellcyte X live fluorescence quantification of IM3 immortalized vascular smooth muscle cells over 7 days using fluorescence-labelled fetuin-A-mRuby3. Cells were cultured in DMEM, 10% FBS, with 10 mM additional Ca and either 10 mM (A-B) or 5 mM additional Pi (C-D) with additional Mg as listed in the figure legend. (A, C): Complete time course of live imaging data. (B, D): First 72 hours of time course, zoomed for readability. Each data series consists of fluorescence quantification of four fields of view per condition with error bars shown (n = 4). Fluorescence intensity of fetuin-A-mRuby3 indicates degree of calcification, with background intensity measured in DMEM. Error bars depict standard deviation with very small errors not visible.

    Techniques Used: Fluorescence, Cell Culture, Imaging, Standard Deviation



    Similar Products

    cellcyte studio software  (Cytena Inc)
    86
    Cytena Inc cellcyte studio software
    <t>Cellcyte</t> X live imaging of IM3 immortalized vascular smooth muscles over 7 days in (A) DMEM, 10% FBS, (B) calcification medium CM (DMEM, 10% FBS, adjusted to 4.3 mM Ca and 3 mM Pi), and (C) endothelial calcification medium ECM-2 (endothelial base medium EBM-2, 10% FBS, adjusted to 4.3 mM total Ca and 3.0 mM total Pi). Movies are time-lapse with frames acquired every three hours. C.Live Tox green, a nuclear stain demarcating dead cells, is visible in green. (D) Quantification of threshold object count in the green channel, indicating the number of dead cells per field of view. Each data series consists of four analyzed fields of view of the same condition. Each measurement was performed in quadruplicate (n = 4). Error bars depict standard deviation.
    Cellcyte Studio Software, supplied by Cytena Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellcyte studio software/product/Cytena Inc
    Average 86 stars, based on 1 article reviews
    cellcyte studio software - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Cellcyte X live imaging of IM3 immortalized vascular smooth muscles over 7 days in (A) DMEM, 10% FBS, (B) calcification medium CM (DMEM, 10% FBS, adjusted to 4.3 mM Ca and 3 mM Pi), and (C) endothelial calcification medium ECM-2 (endothelial base medium EBM-2, 10% FBS, adjusted to 4.3 mM total Ca and 3.0 mM total Pi). Movies are time-lapse with frames acquired every three hours. C.Live Tox green, a nuclear stain demarcating dead cells, is visible in green. (D) Quantification of threshold object count in the green channel, indicating the number of dead cells per field of view. Each data series consists of four analyzed fields of view of the same condition. Each measurement was performed in quadruplicate (n = 4). Error bars depict standard deviation.

    Journal: bioRxiv

    Article Title: Cell-based calcification assays – magnesium overrules calcium phosphate saturation in overall outcome

    doi: 10.64898/2025.12.23.696208

    Figure Lengend Snippet: Cellcyte X live imaging of IM3 immortalized vascular smooth muscles over 7 days in (A) DMEM, 10% FBS, (B) calcification medium CM (DMEM, 10% FBS, adjusted to 4.3 mM Ca and 3 mM Pi), and (C) endothelial calcification medium ECM-2 (endothelial base medium EBM-2, 10% FBS, adjusted to 4.3 mM total Ca and 3.0 mM total Pi). Movies are time-lapse with frames acquired every three hours. C.Live Tox green, a nuclear stain demarcating dead cells, is visible in green. (D) Quantification of threshold object count in the green channel, indicating the number of dead cells per field of view. Each data series consists of four analyzed fields of view of the same condition. Each measurement was performed in quadruplicate (n = 4). Error bars depict standard deviation.

    Article Snippet: The full-frame fluorescence intensity, proportional to fetuin-A-bound mineral deposition, was quantified using Cellcyte Studio software (Cytena) and exported in .csv file format for further analysis.

    Techniques: Imaging, Muscles, Staining, Standard Deviation

    Cellcyte-X live imaging of IM3 immortalized vascular smooth muscle cells over 7 days in DMEM, 10% FBS, with 10 mM added Ca and Pi and (A) 1.25 mM, (B) 2.5 mM, (C) 5 mM, and 10 mM added Mg. Fetuin-A-mRuby staining of calcification is shown in red. Movement of living cells can be seen in , but not in C. The morphology of the calcification can be seen to change with increasing Mg. Lower concentrations of Mg exhibited more rapid onset of calcification along cell boundaries, while higher concentrations exhibited delayed onset followed by sudden and sheet-like calcification across the cell layer. At the highest concentration, 10 mM added Mg, no calcification was observed.

    Journal: bioRxiv

    Article Title: Cell-based calcification assays – magnesium overrules calcium phosphate saturation in overall outcome

    doi: 10.64898/2025.12.23.696208

    Figure Lengend Snippet: Cellcyte-X live imaging of IM3 immortalized vascular smooth muscle cells over 7 days in DMEM, 10% FBS, with 10 mM added Ca and Pi and (A) 1.25 mM, (B) 2.5 mM, (C) 5 mM, and 10 mM added Mg. Fetuin-A-mRuby staining of calcification is shown in red. Movement of living cells can be seen in , but not in C. The morphology of the calcification can be seen to change with increasing Mg. Lower concentrations of Mg exhibited more rapid onset of calcification along cell boundaries, while higher concentrations exhibited delayed onset followed by sudden and sheet-like calcification across the cell layer. At the highest concentration, 10 mM added Mg, no calcification was observed.

    Article Snippet: The full-frame fluorescence intensity, proportional to fetuin-A-bound mineral deposition, was quantified using Cellcyte Studio software (Cytena) and exported in .csv file format for further analysis.

    Techniques: Imaging, Staining, Concentration Assay

    Cellcyte X live fluorescence quantification of IM3 immortalized vascular smooth muscle cells over 7 days using fluorescence-labelled fetuin-A-mRuby3. Cells were cultured in DMEM, 10% FBS, with 10 mM additional Ca and either 10 mM (A-B) or 5 mM additional Pi (C-D) with additional Mg as listed in the figure legend. (A, C): Complete time course of live imaging data. (B, D): First 72 hours of time course, zoomed for readability. Each data series consists of fluorescence quantification of four fields of view per condition with error bars shown (n = 4). Fluorescence intensity of fetuin-A-mRuby3 indicates degree of calcification, with background intensity measured in DMEM. Error bars depict standard deviation with very small errors not visible.

    Journal: bioRxiv

    Article Title: Cell-based calcification assays – magnesium overrules calcium phosphate saturation in overall outcome

    doi: 10.64898/2025.12.23.696208

    Figure Lengend Snippet: Cellcyte X live fluorescence quantification of IM3 immortalized vascular smooth muscle cells over 7 days using fluorescence-labelled fetuin-A-mRuby3. Cells were cultured in DMEM, 10% FBS, with 10 mM additional Ca and either 10 mM (A-B) or 5 mM additional Pi (C-D) with additional Mg as listed in the figure legend. (A, C): Complete time course of live imaging data. (B, D): First 72 hours of time course, zoomed for readability. Each data series consists of fluorescence quantification of four fields of view per condition with error bars shown (n = 4). Fluorescence intensity of fetuin-A-mRuby3 indicates degree of calcification, with background intensity measured in DMEM. Error bars depict standard deviation with very small errors not visible.

    Article Snippet: The full-frame fluorescence intensity, proportional to fetuin-A-bound mineral deposition, was quantified using Cellcyte Studio software (Cytena) and exported in .csv file format for further analysis.

    Techniques: Fluorescence, Cell Culture, Imaging, Standard Deviation